Background: TheAsian corn borer (ACB), Ostriniafurnacalis(Guenée),has become the most damaging insect pest of corn in Asia.However, the lack of genome or transcriptome information heavily hinders our furtherunderstanding of ACB in every aspect at a molecular level and on a genome-wide scale. Here, we usedthe Ion Torrent Personal Genome Machine (PGM) Sequencer to explorethe ACBtranscriptome and to identify relevant genes in response to flubendiamide, showing high selective activity against ACB.
Results:Weobtained 35,430 unigenes, with an average length of 716bp, representing a dramatic expansion ofexisting cDNA sequences available for ACB. These sequences were annotated with Non-redundant Protein (Nr), Gene Ontology (GO), Clusters of Orthologous Groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to better understand their functions. A total of 31cytochrome P450monooxygenases(P450s), 27 carboxyl/cholinesterases (CCEs) and19glutathione S-transferases (GSTs) were manually curated to construct phylogenetic trees, and 25unigenes encoding target proteins (acetylcholinesterase, nicotinic acetylcholine receptor, gamma-aminobutyric acid receptor, glutamate-gated chloride channel, voltage-gated sodium channel and ryanodine receptor) were identified. In addition, we compared and validated the differentially expressed unigenes upon flubendiamide treatment, revealing that the genes for detoxification enzymes(P450s and esterase), calcium signaling pathways and muscle control pathways (twitchin and tropomyosin), immunoglobulin (hemolin), chemosensory protein and heat shock protein 70 were significantly overexpressed inresponse to flubendiamide, while the genes for cuticular protein, proteaseand oxidoreductaseshowedmuch lower expressionlevels.
Conclusion:The obtained transcriptome information provides large genomic resources available for further studies of ACB. The differentially expressed gene data will elucidate the molecular mechanisms of ACBin response to the novel diamideinsecticide, flubendiamide. In particular, these findings will facilitate theidentification of the genes involved in insecticide resistance and the development of new compounds to control the ACB.
论文链接http://www.biomedcentral.com/survey/display/1076296380201990/1514484732201990/SRV2/
