JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY卷: 63  期: 27，页: 6126-6132 DOI: 10.1021/acs.jafc.5b01662 出版年: JUL 15 2015

摘要

The honey bee, Apis mellifera, is commonly used as a test species for the regulatory risk assessment of insect-resistant genetically engineered (IRGE) plants. In the current study, a dietary exposure assay was developed, validated, and used to assess the potential toxicity of Cry1C and Cry2A proteins from Bacillus thuringiensis (Bt) to A. mellifera larvae; Cry1C and Cry2A are produced by different IRGE crops. The assay, Which uses the soybean trypsin inhibitor (SBTI) as a positive control and bovine serum: albumin (BSA) as a negative control, was used to measure the responses Of A. mellifera larvae to high concentrations of Cry1C and Cry2A. Survival was reduced and development was delayed when larvae were fed SBTI (1 mg/g diet) but were unaffected when larvae were fed BSA (400 mu g/g), Cry1C (50 mu g/g), or Cry2A (400 mu g/g). The enzymatic activities of A. mellifera larvae Were not altered and their midgut brush border membranes (BBMs) were not damaged after being fed with diets containing BSA, Cry1C, or Cry2A; however, enzymatic activities were increased and BBMs were damaged when diets contained SBTI. The study confirms that Cry1C and Cry2A have no acute toxicity to A mellifera larvae at concentrations >10 times higher than those detected in pollen from Bt plants.

关键词:nontarget effects; in vitro; tier 1 study; environmental risk assessment; SBTI